Mouse genetics
Mouse Genotyping
Quick DNA isolation for genotyping
Clean DNA isolation with choloroform prep
Toe clipping protocol, Ear punch protocol
Kras LSLG12D
p53
p53 CKO allele
Eu-Myc
miR-34a KO
miR-34b/34c KO
miR-449 KO
miR-34b/34c fl allele
miR-200c/141 KO; miR-200b/200a/429 KO; miR-
200c/141-LacZ fl allele
miR-200c/141:LacZ FLP'd OUT
mTmG
LSL-Cas9 (From Jacks Lab)
dCas9-KRAB
Mouse models
Eu-myc B-lymphoma model
fetal liver collection
adoptive transfer
KrasG12D induced lung adenocarcinoma model
determine Ade-Cre viral titer
intranasal incubation of adeno/lenti virus
Intratracheal injection of lenti/Adeno virus
Lung sample preparation - Histology
Prepare primary lung cancer cell lines
Teratoma generation from ESCs or iPSCs
Superovulation for embryo collection
CRISPR-EZ [protocol] [video]
B6 colony
Histology / IF / In situ / staining analyses
HE using paraffin section
KOS machine processing
Bone paraffin embedding
IF using cryo section - [Jenn] [Yongjin]
IF using paraffin section [Trilogy] [No Trilogy]
IHC using paraffin section
mRNA/ncRNA in situ using cryo section
mRNA/ncRNA in situ using paraffin section
miRNA in situ using cryo section
miRNA in situ using paraffin section
TRAP staining
PAS staining
Trichrome staining
EM preparation
Molecular Biology
General intro on RNA, DNA and protein work
RNA biology
RNA prep (trizol)
microRNA real-time PCR
He Lab protocol - Paul Lin
ABI protocol
mRNA real time PCR
Single embryo/cell real time PCR [ABI kit] [Smart-
seq protocol]
Luciferase assays (for miRNA target validation)
small RNA sequencing
RNA-seq (polyA enriched, strand specific) -Li Lab
small RNA northern [Hannon Lab]
sgRNA design /in vitro transcription / purification
CRISPR-editing in 368 lung cancer cell lines
mRNA Northern
Hammerhead ribozyme
5'RACE 3' RACE [Andrew's protocol] [Clontech protocol]
Protein analysis
Chromatin Immunoprecipitation (ChIP)- Keji Zhao
CHiP data analyses -Paul
Western (with tips for miRNA target studies)
Bradford analysis - Frank Qi
Western protocol - Paul Lin
How to run a best western _Paul
Luciferase assay for promoter activity
Luciferase assay for translation efficiency
Southern [Hannon lab]
OP Puro
Cloning
Golden Gate cloning
Gibson assembly
Mutagenesis protocol - Enbo Ma
Pfu Turbo
DNA analyses
Bisulfate sequencing
[EZ DNA methylation kit], [Jump Start Taq]