CRISPR genome editing in mice
CRISPR-mediated mouse genome editing is typically accomplished by microinjection of Cas9 DNA/RNA and sgRNA into zygotes to achieve editing in one step. We developed a simple and economic electroporation-based strategy, designated as CRISPR RNP Electroporation of Zygotes (CRISPR-EZ), to deliver Cas9/sgRNA ribonucleoproteins (RNPs) into mouse zygotes with 100% efficiency. CRISPR-EZ enables highly efficient and high-throughput genome editing in vivo, with a significant improvement in embryo viability compared with microinjection-based technology
CRISPR-EZ Protocol
Publication on CRISPR-EZ
CRISPR-EZ Video Protocol
More information on the experimental procedure is available upon request by contacting glinhe@gmail.com
CRISPR-mediated mouse genome editing is typically accomplished by microinjection of Cas9 DNA/RNA and sgRNA into zygotes to achieve editing in one step. We developed a simple and economic electroporation-based strategy, designated as CRISPR RNP Electroporation of Zygotes (CRISPR-EZ), to deliver Cas9/sgRNA ribonucleoproteins (RNPs) into mouse zygotes with 100% efficiency. CRISPR-EZ enables highly efficient and high-throughput genome editing in vivo, with a significant improvement in embryo viability compared with microinjection-based technology
CRISPR-EZ Protocol
Publication on CRISPR-EZ
CRISPR-EZ Video Protocol
More information on the experimental procedure is available upon request by contacting glinhe@gmail.com